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The steady-state mean projected area of the total biomass was found to increase signi cantly from 6100 1100 m2 (mean standard error) at 1000 rpm to 16 500 3800 m2 at 550 rpm. Protein production ( -amylase and amyloglucosidase) was found to be independent of agitation speed in the range 550 to 1000 rpm (P/V = 2.2 and 12.6 kW/m3 , respectively) (Figure 18-20), although signi cant changes in mycelial morphology could be measured for similar changes in agitation conditions. This suggests that mycelial morphology in his strain does not directly affect protein production (at a constant dilution rate and therefore, speci c growth rate). Although there is very limited use of continuous culture systems in industry, they are extremely useful research tools since they can give precise information on the in uence of a single variable. However, it was important to verify the results in fed-batch fermentations at industrially realistic conditions of biomass concentration and speci c power input. 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